/* faidx.c -- FASTA random access. Copyright (C) 2008, 2009, 2013-2016 Genome Research Ltd. Portions copyright (C) 2011 Broad Institute. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include "htslib/bgzf.h" #include "htslib/faidx.h" #include "htslib/hfile.h" #include "htslib/khash.h" #include "htslib/kstring.h" typedef struct { int32_t line_len, line_blen; int64_t len; uint64_t offset; } faidx1_t; KHASH_MAP_INIT_STR(s, faidx1_t) struct __faidx_t { BGZF *bgzf; int n, m; char **name; khash_t(s) *hash; }; #ifndef kroundup32 #define kroundup32(x) (--(x), (x)|=(x)>>1, (x)|=(x)>>2, (x)|=(x)>>4, (x)|=(x)>>8, (x)|=(x)>>16, ++(x)) #endif static inline int fai_insert_index(faidx_t *idx, const char *name, int len, int line_len, int line_blen, uint64_t offset) { if (!name) { fprintf(stderr, "[fai_build_core] malformed line\n"); return -1; } char *name_key = strdup(name); int absent; khint_t k = kh_put(s, idx->hash, name_key, &absent); faidx1_t *v = &kh_value(idx->hash, k); if (! absent) { fprintf(stderr, "[fai_build_core] ignoring duplicate sequence \"%s\" at byte offset %"PRIu64"\n", name, offset); free(name_key); return 0; } if (idx->n == idx->m) { char **tmp; idx->m = idx->m? idx->m<<1 : 16; if (!(tmp = (char**)realloc(idx->name, sizeof(char*) * idx->m))) { fprintf(stderr, "[fai_build_core] out of memory\n"); return -1; } idx->name = tmp; } idx->name[idx->n++] = name_key; v->len = len; v->line_len = line_len; v->line_blen = line_blen; v->offset = offset; return 0; } faidx_t *fai_build_core(BGZF *bgzf) { kstring_t name = { 0, 0, NULL }; int c; int line_len, line_blen, state; int l1, l2; faidx_t *idx; uint64_t offset; int64_t len; idx = (faidx_t*)calloc(1, sizeof(faidx_t)); idx->hash = kh_init(s); len = line_len = line_blen = -1; state = 0; l1 = l2 = -1; offset = 0; while ( (c=bgzf_getc(bgzf))>=0 ) { if (c == '\n') { // an empty line if (state == 1) { offset = bgzf_utell(bgzf); continue; } else if ((state == 0 && len < 0) || state == 2) continue; else if (state == 0) { state = 2; continue; } } if (c == '>') { // fasta header if (len >= 0) { if (fai_insert_index(idx, name.s, len, line_len, line_blen, offset) != 0) goto fail; } name.l = 0; while ((c = bgzf_getc(bgzf)) >= 0) if (! isspace(c)) kputc_(c, &name); else if (name.l > 0 || c == '\n') break; kputsn("", 0, &name); if ( c<0 ) { fprintf(stderr, "[fai_build_core] the last entry has no sequence\n"); goto fail; } if (c != '\n') while ( (c=bgzf_getc(bgzf))>=0 && c != '\n'); state = 1; len = 0; offset = bgzf_utell(bgzf); } else { if (state == 3) { fprintf(stderr, "[fai_build_core] inlined empty line is not allowed in sequence '%s'.\n", name.s); goto fail; } if (state == 2) state = 3; l1 = l2 = 0; do { ++l1; if (isgraph(c)) ++l2; } while ( (c=bgzf_getc(bgzf))>=0 && c != '\n'); if (state == 3 && l2) { fprintf(stderr, "[fai_build_core] different line length in sequence '%s'.\n", name.s); goto fail; } ++l1; len += l2; if (state == 1) line_len = l1, line_blen = l2, state = 0; else if (state == 0) { if (l1 != line_len || l2 != line_blen) state = 2; } } } if (len >= 0) { if (fai_insert_index(idx, name.s, len, line_len, line_blen, offset) != 0) goto fail; } else { goto fail; } free(name.s); return idx; fail: free(name.s); fai_destroy(idx); return NULL; } void fai_save(const faidx_t *fai, FILE *fp) { khint_t k; int i; for (i = 0; i < fai->n; ++i) { faidx1_t x; k = kh_get(s, fai->hash, fai->name[i]); x = kh_value(fai->hash, k); #ifdef _WIN32 fprintf(fp, "%s\t%d\t%ld\t%d\t%d\n", fai->name[i], (int)x.len, (long)x.offset, (int)x.line_blen, (int)x.line_len); #else fprintf(fp, "%s\t%d\t%lld\t%d\t%d\n", fai->name[i], (int)x.len, (long long)x.offset, (int)x.line_blen, (int)x.line_len); #endif } } static faidx_t *fai_read(FILE *fp, const char *fname) { faidx_t *fai; char *buf, *p; int len, line_len, line_blen; #ifdef _WIN32 long offset; #else long long offset; #endif fai = (faidx_t*)calloc(1, sizeof(faidx_t)); fai->hash = kh_init(s); buf = (char*)calloc(0x10000, 1); while (fgets(buf, 0x10000, fp)) { for (p = buf; *p && isgraph(*p); ++p); *p = 0; ++p; #ifdef _WIN32 sscanf(p, "%d%ld%d%d", &len, &offset, &line_blen, &line_len); #else sscanf(p, "%d%lld%d%d", &len, &offset, &line_blen, &line_len); #endif if (fai_insert_index(fai, buf, len, line_len, line_blen, offset) != 0) { free(buf); return NULL; } } free(buf); if (ferror(fp)) { fprintf(stderr, "[fai_load] error while reading \"%s\": %s\n", fname, strerror(errno)); fai_destroy(fai); return NULL; } return fai; } void fai_destroy(faidx_t *fai) { int i; for (i = 0; i < fai->n; ++i) free(fai->name[i]); free(fai->name); kh_destroy(s, fai->hash); if (fai->bgzf) bgzf_close(fai->bgzf); free(fai); } int fai_build(const char *fn) { char *str; BGZF *bgzf; FILE *fp; faidx_t *fai; str = (char*)calloc(strlen(fn) + 5, 1); sprintf(str, "%s.fai", fn); bgzf = bgzf_open(fn, "r"); if ( !bgzf ) { fprintf(stderr, "[fai_build] fail to open the FASTA file %s\n",fn); free(str); return -1; } if ( bgzf->is_compressed ) bgzf_index_build_init(bgzf); fai = fai_build_core(bgzf); if ( !fai ) { if ( bgzf->is_compressed && bgzf->is_gzip ) fprintf(stderr,"Cannot index files compressed with gzip, please use bgzip\n"); bgzf_close(bgzf); free(str); return -1; } if ( bgzf->is_compressed ) { if (bgzf_index_dump(bgzf, fn, ".gzi") < 0) { fprintf(stderr, "[fai_build] fail to make bgzf index %s.gzi\n", fn); fai_destroy(fai); free(str); return -1; } } if (bgzf_close(bgzf) < 0) { fprintf(stderr, "[fai_build] Error on closing %s\n", fn); fai_destroy(fai); free(str); return -1; } fp = fopen(str, "wb"); if ( !fp ) { fprintf(stderr, "[fai_build] fail to write FASTA index %s\n",str); fai_destroy(fai); free(str); return -1; } fai_save(fai, fp); fclose(fp); free(str); fai_destroy(fai); return 0; } static FILE *download_and_open(const char *fn) { const int buf_size = 1 * 1024 * 1024; uint8_t *buf; FILE *fp; hFILE *fp_remote; const char *url = fn; const char *p; int l = strlen(fn); for (p = fn + l - 1; p >= fn; --p) if (*p == '/') break; fn = p + 1; // First try to open a local copy fp = fopen(fn, "r"); if (fp) return fp; // If failed, download from remote and open fp_remote = hopen(url, "rb"); if (fp_remote == 0) { fprintf(stderr, "[download_from_remote] fail to open remote file %s\n",url); return NULL; } if ((fp = fopen(fn, "wb")) == 0) { fprintf(stderr, "[download_from_remote] fail to create file in the working directory %s\n",fn); hclose_abruptly(fp_remote); return NULL; } buf = (uint8_t*)calloc(buf_size, 1); while ((l = hread(fp_remote, buf, buf_size)) > 0) fwrite(buf, 1, l, fp); free(buf); fclose(fp); if (hclose(fp_remote) != 0) fprintf(stderr, "[download_from_remote] fail to close remote file %s\n", url); return fopen(fn, "r"); } faidx_t *fai_load(const char *fn) { char *str; FILE *fp; faidx_t *fai; str = (char*)calloc(strlen(fn) + 5, 1); sprintf(str, "%s.fai", fn); if (hisremote(str)) { fp = download_and_open(str); if ( !fp ) { fprintf(stderr, "[fai_load] failed to open remote FASTA index %s\n", str); free(str); return 0; } } else fp = fopen(str, "rb"); if (fp == 0) { fprintf(stderr, "[fai_load] build FASTA index.\n"); if (fai_build(fn) < 0) { free(str); return 0; } fp = fopen(str, "rb"); if (fp == 0) { fprintf(stderr, "[fai_load] failed to open FASTA index: %s\n", strerror(errno)); free(str); return 0; } } fai = fai_read(fp, str); fclose(fp); free(str); if (fai == NULL) { return NULL; } fai->bgzf = bgzf_open(fn, "rb"); if (fai->bgzf == 0) { fprintf(stderr, "[fai_load] fail to open FASTA file.\n"); return 0; } if ( fai->bgzf->is_compressed==1 ) { if ( bgzf_index_load(fai->bgzf, fn, ".gzi") < 0 ) { fprintf(stderr, "[fai_load] failed to load .gzi index: %s[.gzi]\n", fn); fai_destroy(fai); return NULL; } } return fai; } char *fai_fetch(const faidx_t *fai, const char *str, int *len) { char *s; int c, i, l, k, name_end; khiter_t iter; faidx1_t val; khash_t(s) *h; int beg, end; beg = end = -1; h = fai->hash; name_end = l = strlen(str); s = (char*)malloc(l+1); // remove space for (i = k = 0; i < l; ++i) if (!isspace(str[i])) s[k++] = str[i]; s[k] = 0; l = k; // determine the sequence name for (i = l - 1; i >= 0; --i) if (s[i] == ':') break; // look for colon from the end if (i >= 0) name_end = i; if (name_end < l) { // check if this is really the end int n_hyphen = 0; for (i = name_end + 1; i < l; ++i) { if (s[i] == '-') ++n_hyphen; else if (!isdigit(s[i]) && s[i] != ',') break; } if (i < l || n_hyphen > 1) name_end = l; // malformated region string; then take str as the name s[name_end] = 0; iter = kh_get(s, h, s); if (iter == kh_end(h)) { // cannot find the sequence name iter = kh_get(s, h, str); // try str as the name if (iter == kh_end(h)) { *len = 0; free(s); return 0; } else s[name_end] = ':', name_end = l; } } else iter = kh_get(s, h, str); if(iter == kh_end(h)) { fprintf(stderr, "[fai_fetch] Warning - Reference %s not found in FASTA file, returning empty sequence\n", str); free(s); *len = -2; return 0; }; val = kh_value(h, iter); // parse the interval if (name_end < l) { for (i = k = name_end + 1; i < l; ++i) if (s[i] != ',') s[k++] = s[i]; s[k] = 0; beg = atoi(s + name_end + 1); for (i = name_end + 1; i != k; ++i) if (s[i] == '-') break; end = i < k? atoi(s + i + 1) : val.len; if (beg > 0) --beg; } else beg = 0, end = val.len; if (beg >= val.len) beg = val.len; if (end >= val.len) end = val.len; if (beg > end) beg = end; free(s); // now retrieve the sequence int ret = bgzf_useek(fai->bgzf, val.offset + beg / val.line_blen * val.line_len + beg % val.line_blen, SEEK_SET); if ( ret<0 ) { *len = -1; fprintf(stderr, "[fai_fetch] Error: fai_fetch failed. (Seeking in a compressed, .gzi unindexed, file?)\n"); return NULL; } l = 0; s = (char*)malloc(end - beg + 2); while ( (c=bgzf_getc(fai->bgzf))>=0 && l < end - beg ) if (isgraph(c)) s[l++] = c; s[l] = '\0'; *len = l; return s; } int faidx_fetch_nseq(const faidx_t *fai) { return fai->n; } int faidx_nseq(const faidx_t *fai) { return fai->n; } const char *faidx_iseq(const faidx_t *fai, int i) { return fai->name[i]; } int faidx_seq_len(const faidx_t *fai, const char *seq) { khint_t k = kh_get(s, fai->hash, seq); if ( k == kh_end(fai->hash) ) return -1; return kh_val(fai->hash, k).len; } char *faidx_fetch_seq(const faidx_t *fai, const char *c_name, int p_beg_i, int p_end_i, int *len) { int l, c; khiter_t iter; faidx1_t val; char *seq=NULL; // Adjust position iter = kh_get(s, fai->hash, c_name); if (iter == kh_end(fai->hash)) { *len = -2; fprintf(stderr, "[fai_fetch_seq] The sequence \"%s\" not found\n", c_name); return NULL; } val = kh_value(fai->hash, iter); if(p_end_i < p_beg_i) p_beg_i = p_end_i; if(p_beg_i < 0) p_beg_i = 0; else if(val.len <= p_beg_i) p_beg_i = val.len - 1; if(p_end_i < 0) p_end_i = 0; else if(val.len <= p_end_i) p_end_i = val.len - 1; // Now retrieve the sequence int ret = bgzf_useek(fai->bgzf, val.offset + p_beg_i / val.line_blen * val.line_len + p_beg_i % val.line_blen, SEEK_SET); if ( ret<0 ) { *len = -1; fprintf(stderr, "[fai_fetch_seq] Error: fai_fetch failed. (Seeking in a compressed, .gzi unindexed, file?)\n"); return NULL; } l = 0; seq = (char*)malloc(p_end_i - p_beg_i + 2); while ( (c=bgzf_getc(fai->bgzf))>=0 && l < p_end_i - p_beg_i + 1) if (isgraph(c)) seq[l++] = c; seq[l] = '\0'; *len = l; return seq; } int faidx_has_seq(const faidx_t *fai, const char *seq) { khiter_t iter = kh_get(s, fai->hash, seq); if (iter == kh_end(fai->hash)) return 0; return 1; }