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ABI_Instrument-1327-01031XX UnitID=7;CPUBoard=ECPU550;ArraySize=16;SerialNumber=1327-010; 3130xl 1512128 Jul 15, 2016
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 091757305f8d11e9bfe80023ae57e8b0StdSeq50_POP7-optimized_13130POP7_BDTv3-KB-Denovo_v5.2
KB.bcpThis is the default analysis protocol for 3130 for BDTv3 and POP7 polymer using the KB Basecaller. Phred files will be created after user analyzes and saves samples KB_3130_POP7_BDTv3.mobfalsetruefalsetrue10.00KB_3130_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACbAAAAAP//////////////////////////////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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
StdSeq50_POP7-optimized_1
StdSeq50_POP7-optimized_11FastSeq50_POP71For vector/pl >5000 n.b.Oven_Temperature60Temperature_Tolerance3Oven_Wait_Time1200Array_Heater_Temperature50Poly_Fill_Vol7300Cap_Fill_Vol2970DC_Cap16_EPS_ARC_SELF8Max_Current300Current_Stability5.0Current_Tolerance100Run_Current100Voltage_Tolerance0.6PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.6DC_Injection_Time30Voltage_Number_Of_Steps40Voltage_Step_Interval15DC_Data_Delay480Run_Voltage8.5DC_Run_Time6000First_ReadOut_Time200Second_ReadOut_Time200Exposure_Time0DC_RS_Plate_TypeA 12 8DC_RS_CSDatacap1 A1DC_RS_Binning_PatternDC_Scale_Divisor8.0DC_Down_Sample1DC_Laser_Power_Setting15.0StdSeq50_POP7_optimized_1 Run_ABI_Instrument_2019-04-15_07-56_4685 BIOCAD_SEQ_Results_Group 1301_ 1301_ 96-Well pEE-CK_TNR014_1b-DD_RP1_H2_seqFKB_3130_POP7_BDTv3.mob 3130POP7_BDTv3-KB-Denovo_v5.2h2 Z K \ Jd
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?%?@@SKB.bcpKB_3130_POP7_BDTv3.mobtermNNNNNNNNNN
" . :
" . : NNNNNBCPFileName=KB.bcp;MobilityFileName=KB_3130_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.02019-04-16 02:55:15 +03:00AEPt = AEPt = APFN ; APXV 2 APrN ס APrV 1 APrX > > ARTN v ASPF ASPt q ASPt q B1Pt q B1Pt q BCTS CTID CTNM CTOw ok CTTL ] CpEP DATA u DATA u DATA u DATA u ! DATA ^ / DATA ^ DATA ^ DATA ^ I DATA =
z Y DATA
=
z ns DATA =
z DATA =
z b DCHT 2 DSam DySN > Dye# DyeN I DyeN N DyeN S DyeN X DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG > > InSc InVt @ LANE LAST c c [ LIMS ! ! f LNTD 2 LsrP : MCHN MODF ׇ MODL 3100 NAVG NLNE NOIS P1AM
. P1RL
$ P1WD
8 P2AM
L P2BA V P2RL
B PBAS PBAS PCON PCON PDMF $ PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm RGOw PN RMXV 1 RMdN RMdV 1 RMdX } } RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT & RUNT RUNT / RUNT
Rate RunN ) ) S/N% SCAN u SMED
SMLt SMPL SPAC Vu2 SPAC SPAC Vu2 SVER 3.0 SVER SVER , , ֧ Scal A Scan u TUBE H2 Tmpr < User ok phAR phCH phDY big phQL c phTR phTR 3130POP7_BDTv3-KB-Denovo_v5.2
KB.bcpThis is the default analysis protocol for 3130 for BDTv3 and POP7 polymer using the KB Basecaller. Phred files will be created after user analyzes and saves samples KB_3130_POP7_BDTv3.mobfalsetruefalsetrue10.00KB_3130_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACbAAAAAP//////////////////////////////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16491649-13.40361215623falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
AEPt = AEPt = APFN ; APXV 2 APrN APrV 2 APrX O O ARTN v ASPF ASPt q ASPt q B1Pt q B1Pt q BCTS CTID CTNM CTOw ok CTTL ] CpEP DATA u DATA u DATA u DATA u ! DATA ^ / DATA ^ DATA ^ DATA ^ I DATA =
z Y DATA
=
z ns DATA =
z DATA =
z b DCHT 2 DSam DySN > Dye# DyeN I DyeN N DyeN S DyeN X DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG > > InSc InVt @ LANE LAST c c [ LIMS ! ! f LNTD 2 LsrP : MCHN MODF ׇ MODL 3100 NAVG NLNE NOIS P1AM
. P1RL
$ P1WD
8 P2AM
L P2BA V P2RL
B PBAS PBAS PCON PCON PDMF $ PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm RGOw PN RMXV 1 RMdN RMdV 1 RMdX } } RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT & RUNT RUNT / RUNT
Rate RunN ) ) S/N% SCAN u SMED
SMLt SMPL SPAC Vu2 SPAC SPAC Vu2 SVER 3.0 SVER SVER , , ֧ Scal A Scan u TUBE H2 Tmpr < User ok phAR phCH phDY big phQL c phTR phTR pEE-CK_TNR014_1b-DD_RP1_H2_seqFNNNNN NNNNN
" . :AEPt = AEPt = APFN ; APXV 2 APrN APrV 2 APrX O O ARTN v ASPF ASPt q ASPt q B1Pt q B1Pt q BCTS CTID CTNM CTOw ok CTTL ] CpEP DATA u DATA u DATA u DATA u ! DATA ^ / DATA ^ DATA ^ DATA ^ I DATA =
z Y DATA
=
z ns DATA =
z DATA =
z b DCHT 2 DSam DySN > Dye# DyeN I DyeN N DyeN S DyeN X DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG > > InSc InVt @ LANE LAST c c [ LIMS ! ! f LNTD 2 LsrP : MCHN MODF ׇ MODL 3100 NAVG NLNE NOIS P1AM
. P1RL
$ P1WD
8 P2AM
L P2BA V P2RL
B PBAS
o PBAS
y PCON
t PCON
~ PDMF $ PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm RGOw PN RMXV 1 RMdN RMdV 1 RMdX } } RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT & RUNT RUNT / RUNT
Rate RevC RunN ) ) S/N% SCAN u SMED
SMLt SMPL
O SPAC Vu2 SPAC SPAC Vu2 SVER 3.0 SVER SVER , , ֧ Scal A Scan u TUBE H2 Tmpr < User ok phAR phCH phDY big phQL c phTR phTR