LOCUS Exported 6951 bp DNA circular SYN 04-MAR-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pIntA-GFPst-CK SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6951) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 14, 2021 from SnapGene Viewer 5.3.2 https://www.snapgene.com COMMENT ORIGDB|GenBank FEATURES Location/Qualifiers source 1..6951 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin join(1,6551..6951) /label=pUCorigin and also a multiline property /note="/vntifkey=33" enhancer 449..858 /label=cmv enhanser /label=cmv\enhanser /note="/vntifkey=9" misc_feature 859..984 /label=hCMV promoter /label=hCMV\promoter /note="/vntifkey=21" intron 1012..1919 /label=IntronA /note="/vntifkey=15" primer_bind 1502..1522 /label=inv olig1 misc_feature 1945..1953 /label=Kozak /note="/vntifkey=21" misc_feature 1954..2010 /label=Leader IgK /note="Leader IgK" /note="/ugene_name=Leader\ IgK" /note="/vntifkey=21" misc_feature 1954..1956 /label=START /note="START" /note="/ugene_name=START" /note="/vntifkey=21" misc_feature 2011..3739 /label=GFP stuffer /note="GFP stuffer" /note="/ugene_name=GFP\ stuffer" /note="/vntifkey=21" misc_feature 3740..4060 /label=CK /note="CK" /note="/ugene_name=CK" /note="/vntifkey=21" misc_feature 4061..4066 /label=STOP /note="STOP" /note="/ugene_name=STOP" /note="/vntifkey=21" misc_feature 4079..4317 /gene="SV40_PA term" /label=SV40_PA term /label=SV40_PA\term /note="/vntifkey=21" primer_bind 4350..4370 /label=pEE_Clab rep_origin 4582..4975 /label=EBV ori /label=EBV\ori /note="/vntifkey=33" CDS 5543..6403 /codon_start=1 /label=AmpR /note="/vntifkey=4" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" BASE COUNT 1802 a 1764 c 1718 g 1840 t ORIGIN 1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 361 gcgcagcgag tcagtgagcg aggaagcgta catttatatt ggctcatgtc caatatgacc 421 gccatgttga cattgattat tgactagacc gcgttacata acttacggta aatggcccgc 481 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 541 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 601 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 661 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 721 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 781 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 841 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 901 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 961 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 1021 gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg cggattcccc 1081 gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc ttggcttctt 1141 atgcatgcta tactgttttt ggcttggggt ctatacaccc ccgcttcctc atgttatagg 1201 tgatggtata gcttagccta taggtgtggg ttattgacca ttattgacca ctcccctatt 1261 ggtgacgata ctttccatta ctaatccata acatggctct ttgccacaac tctctttatt 1321 ggctatatgc caatacactg tccttcagag actgacacgg actctgtatt tttacaggat 1381 ggggtctcat ttattattta caaattcaca tatacaacac caccgtcccc agtgcccgca 1441 gtttttatta aacataacgt gggatctcca cgcgaatctc gggtacgtgt tccggacatg 1501 ggctcatctc cggtagcggc ggagcttcta catccgagcc ctgctcccat gcctccagcg 1561 actcatggtc gctcggcagc tccttgctcc taacagtgga ggccagactt aggcacagca 1621 cgatgcccac caccaccagt gtgccgcaca aggccgtggc ggtagggtat gtgtctgaaa 1681 atgagctcgg ggagcgggct tgcaccgctg acgcatttgg aagacttaag gcagcggcag 1741 aagaagatgc aggcagctga gttgttgtgt tctgataaga gtcagaggta actcccgttg 1801 cggtgctgtt aacggtggag ggcagtgtag tctgagcagt actcgttgct gccgcgcgcg 1861 ccaccagaca taatagctga cagactaaca gactgttcct ttccatgggt cttttctgca 1921 gtcaccgtcc ttgacacgaa gcttgccgcc accatggaga ccgacaccct gctgctgtgg 1981 gtgctgctgc tgtgggtgcc cgggtcgacg aagagctcat gagcggatac atatttgaat 2041 gtatttagaa aaataaacaa ataggggtca gtgttacaac caattaacca attctgaaca 2101 ttatcgcgag cccatttata cctgaatatg gctcataaca ccccttgttt gcctggcggc 2161 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 2221 gatggtagtg tggggactcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg 2281 aaaggctcag tcgaaagact gggcctttcg cccgggctaa ttatggggtg tcgcccttgg 2341 ggtgagaccc tcgagtgtac agaattctta ctgatacgtg tccagatcaa ccgctttcac 2401 gacctctacc agacacatgt gatcacggcg ctcgtcgcgg tctttgctca gtttggtgtg 2461 gtaggtaatg tgatgataac gcgggatatg cactgccgcg gagcccgcca acggacgatt 2521 catttggctg catttggtaa ccagtttttc ggtcacacct tcaatatcgt acgcctggtt 2581 gaactcaacg cggatgccat tgttaacggt gtcaggcaga atatacagaa tgcttggcgg 2641 gcattggaat gcaacgttct tacgcagaat gtgaccgtct ttcttaaagt tctcaccagt 2701 cagcgtgaca cgattgtaga tagaaccgcg ttcgtaggta accatagcac gcgtcttgta 2761 cacgccgtcg ccttcgaagc tgatggtacg ctcttgggta taaccttccg gcatggcgct 2821 cttaaagaaa tccttgatgt ggctcgggta cttgcgaaac actgaacacc gtagctcagg 2881 gtgctcacca gggttgccca cgggacccgg caggtcgccc gtagtgcaga tgtatttcgc 2941 tttaatggta cccgtggtcg cgtcacccgg taccctcgcc tttaatgata aatttcatac 3001 cttcgacgtc gccttccagt tcggtgatat acgggatctc tttctcaaac agttttgcac 3061 cttccgtcaa tgccgtcata tgtttacctc ctaaggtctc gaaaagttaa acaaaattat 3121 ttctaaaggg aaaccgttgt ggaattgtga gcgctcacaa ttccacatat tataattgtt 3181 atccgctcac aaagcaaata aatttttcat gatttcactg tgcatgaagc tcgtaattgt 3241 tatccgctca caattaaggg cgacacaaaa tttattctaa atgataataa atactgataa 3301 catcttatag tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa 3361 aactgatttt ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag 3421 aaatattgta tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca 3481 tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt 3541 taaataattc tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct 3601 ctttccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg 3661 attaacgatt actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt 3721 tttacaacac aagctcttcc gtacggtggc tgcaccatct gtcttcatct tcccgccatc 3781 tgatgagcag ttgaaatctg gaactgcctc tgttgtgtgc ctgctgaata acttctatcc 3841 cagagaggcc aaagtacagt ggaaggtgga taacgccctc caatcgggta actcccagga 3901 gagtgtcaca gagcaggaca gcaaggacag cacctacagc ctcagcagca ccctgacgct 3961 gagcaaagca gactacgaga aacacaaagt ctacgcctgc gaagtcaccc atcagggcct 4021 gagctcgccc gtcacaaaga gcttcaacag gggagagtgt taatagtcta gacctaggtg 4081 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 4141 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 4201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 4261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaga 4321 tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg 4381 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact 4441 gaaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc 4501 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa 4561 agggtcctaa ggaacagcga tgtaggtggg cgggccaaga taggggcgcg attgctgcga 4621 tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc 4681 atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac 4741 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata ggctatccta 4801 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta 4861 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta 4921 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta 4981 atagagatta gggtagtata tgctatccta atttatatct gggtagcata tactacccaa 5041 atatctggat agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct 5101 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct 5161 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct 5221 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct 5281 gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa ttaattcttg 5341 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 5401 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 5461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 5521 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 5581 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 5641 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 5701 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 5761 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 5821 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 5881 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 5941 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 6001 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 6061 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac 6121 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 6181 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 6241 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 6301 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 6361 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 6421 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 6481 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 6541 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 6601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 6661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 6721 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 6781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 6901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat a //