LOCUS pAAV-GFP-CellBio 5374 bp ds-DNA circular SYN 15-AUG-2016 DEFINITION Saccharomyces cerevisiae TCP1-beta gene, partial cds, and Axl2p (AXL2) and Rev7p (REV7) genes, complete cds. ACCESSION U49845 VERSION U49845.1 GI:1293613 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct synthetic DNA construct hmmm interesting REFERENCE 1 (bases 1 to 5374) AUTHORS avprok TITLE Direct Submission JOURNAL Exported Monday, August 15, 2016 from SnapGene 3.1.4 to Vector NTI(R) format http://www.snapgene.com REFERENCE 2 (bases 1 to 5028) AUTHORS Roemer,T., Madden,K., Chang,J. and Snyder,M. TITLE Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein JOURNAL Genes Dev. 10 (7), 777-793 (1996) PUBMED 8846915 REFERENCE 3 (bases 1 to 5028) AUTHORS Roemer,T. TITLE Direct Submission JOURNAL Submitted (22-FEB-1996) Terry Roemer, Biology, Yale University, New Haven, CT, USA COMMENT VNTDATE|745891200| COMMENT VNTDBDATE|778204800| COMMENT VNTNAME|pAAV-GFP-CellBioLab| COMMENT VNTAUTHORNAME|avprok| FEATURES Location/Qualifiers misc_feature 1..130 /label=Right ITR enhancer 206..509 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 510..712 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 805..1297 /label=Human beta-globin Intron CDS 1320..2036 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" repeat_region complement(2637..2777) /label=Left ITR /note="inverted terminal repeat of adeno-associated virus serotype 2 oooooo" /prop=1 ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag 181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgt 301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 601 gtttgttttg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 661 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt 721 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg 781 ggaccgatcc agcctccgcg gattcgaatc ccggccggga acggtgcatt ggaacgcgga 841 ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc acaaaaaatg 901 ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt 961 tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac 1021 agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat ttctgcatat 1081 aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc cagctaccat 1141 tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag ctaggccctt 1201 ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc 1261 tgtgtgctgg cccatcactt tggcaaagaa ttgggattcg aacatcgatt gaattctgaa 1321 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 1381 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 1441 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 1501 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 1561 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 1621 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 1681 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 1741 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 1801 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 1861 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 1921 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 1981 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtact 2041 cagatctcga gctcaagtag ggatcctcta gagtcgacct gcagaagctt gcctcgagca 2101 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg 2161 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca 2221 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca 2281 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga 2341 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc 2401 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt 2461 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat 2521 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg 2581 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga 2641 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg 2701 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc 2761 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 2821 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg 2881 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2941 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 3001 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3061 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3121 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3181 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg 3241 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 3301 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc 3361 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 3421 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 3481 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 3541 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 3601 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 3661 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 3721 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 3781 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 3841 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 3901 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 3961 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 4021 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 4081 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 4141 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 4201 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 4261 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 4321 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4381 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 4441 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 4501 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 4561 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 4621 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 4681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 4741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 4861 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 5041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 5101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 5161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 5221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 5281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5341 ttcctggcct tttgctggcc ttttgctcac atgt //