LOCUS P2-32_pIntA-TRBV5-1_J1-1-Fc-lama-knob-EPEA 5960 bp circular 27-NOV-2020 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-13020134| COMMENT VNTDBDATE|-13020134| COMMENT LSOWNER| COMMENT VNTNAME|pIntA-mIgK-GFP-stuffer-KpnI-EPEA| COMMENT VNTAUTHORNAME|Demo User| BASE COUNT 1711 a 1577 c 1543 g 1830 t EMBL_ID P1-79_pIntA-mIgK-GFP-stuffer-KpnI-EPEA 5945 bp circular 14-OCT-2020 EMBL_ID P1-79_pIntA-mIgK-GFP-stuffer-KpnI-EPEA 6661 bp circular 29-JAN-2020 EMBL_ID pIntA-mIgK-GFP-s 6661 bp DNA circular 4-OCT-2019 EMBL_ID P1-79_pIntA-mIgK-GFP-stuffer-KpnI-EPEA 5945 bp circular 17-SEP-2020 EMBL_ID P2-32_pIntA-TRBV5-1_J1-1-Fc-lama-knob-EPEA 5945 bp circular 19-OCT-2020 UNIMARK P2-32 pIntA-TRBV5-1_J1-1-Fc-lama-knob-EPEA annotations P2-32 pIntA-TRBV5-1_J1-1-Fc-lama-knob-EPEA FEATURES Location/Qualifiers enhancer 449..858 /label="cmv enhanser" /vntifkey=9 misc_feature 859..984 /label="hCMV promoter" /vntifkey=21 intron 1012..1919 /label="IntronA" /vntifkey=15 misc_feature 1948..1953 /label="Kozak" /vntifkey=21 misc_feature 1954..2013 /label="mIgK Leader" /vntifkey=21 misc_feature 1954..1956 /label="START" /vntifkey=21 misc_feature 2014..2355 /ugene_name="TRBV5-1\ J1-6" misc_feature 2356..2370 /label="human hinge" /vntifkey=21 /ugene_name="human\ hinge" misc_feature 2371..3042 /label="Fc-lama-knob" /vntifkey=21 /ugene_name="Fc-lama-knob" misc_feature 2755..2757 /label="G130C" /vntifkey=21 /ugene_name="G130C" misc_feature 2791..2793 /label="W" /vntifkey=21 /ugene_name="W" misc_feature 3043..3069 /label="EPEA-tag" /vntifkey=21 misc_feature 3044..3049 /label="KpnI added" /vntifkey=21 misc_feature 3070..3075 /label="STOP" /vntifkey=21 misc_feature 3088..3326 /gene="SV40_PA term" /label="SV40_PA term" /vntifkey=21 rep_origin 3591..3984 /label="EBV ori" /vntifkey=33 CDS 4552..5412 /label="AmpR" /vntifkey=4 ORIGIN 1 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 61 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 121 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 181 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 241 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 301 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 361 GCGCAGCGAG TCAGTGAGCG AGGAAGCGTA CATTTATATT GGCTCATGTC CAATATGACC 421 GCCATGTTGA CATTGATTAT TGACTAGACC GCGTTACATA ACTTACGGTA AATGGCCCGC 481 CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG 541 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC 601 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG 661 GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC 721 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA 781 ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA 841 ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG 901 CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC 961 GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC CTCCATAGAA 1021 GACACCGGGA CCGATCCAGC CTCCGCGGCC GGGAACGGTG CATTGGAACG CGGATTCCCC 1081 GTGCCAAGAG TGACGTAAGT ACCGCCTATA GAGTCTATAG GCCCACCCCC TTGGCTTCTT 1141 ATGCATGCTA TACTGTTTTT GGCTTGGGGT CTATACACCC CCGCTTCCTC ATGTTATAGG 1201 TGATGGTATA GCTTAGCCTA TAGGTGTGGG TTATTGACCA TTATTGACCA CTCCCCTATT 1261 GGTGACGATA CTTTCCATTA CTAATCCATA ACATGGCTCT TTGCCACAAC TCTCTTTATT 1321 GGCTATATGC CAATACACTG TCCTTCAGAG ACTGACACGG ACTCTGTATT TTTACAGGAT 1381 GGGGTCTCAT TTATTATTTA CAAATTCACA TATACAACAC CACCGTCCCC AGTGCCCGCA 1441 GTTTTTATTA AACATAACGT GGGATCTCCA CGCGAATCTC GGGTACGTGT TCCGGACATG 1501 GGCTCATCTC CGGTAGCGGC GGAGCTTCTA CATCCGAGCC CTGCTCCCAT GCCTCCAGCG 1561 ACTCATGGTC GCTCGGCAGC TCCTTGCTCC TAACAGTGGA GGCCAGACTT AGGCACAGCA 1621 CGATGCCCAC CACCACCAGT GTGCCGCACA AGGCCGTGGC GGTAGGGTAT GTGTCTGAAA 1681 ATGAGCTCGG GGAGCGGGCT TGCACCGCTG ACGCATTTGG AAGACTTAAG GCAGCGGCAG 1741 AAGAAGATGC AGGCAGCTGA GTTGTTGTGT TCTGATAAGA GTCAGAGGTA ACTCCCGTTG 1801 CGGTGCTGTT AACGGTGGAG GGCAGTGTAG TCTGAGCAGT ACTCGTTGCT GCCGCGCGCG 1861 CCACCAGACA TAATAGCTGA CAGACTAACA GACTGTTCCT TTCCATGGGT CTTTTCTGCA 1921 GTCACCGTCC TTGACACGAA GCTTGCCGCC ACCATGGAGA CCGACACCCT GCTGCTGTGG 1981 GTGCTGCTGC TGTGGGTGCC CGGGTCGACC GGTGCAGGTG TCACACAGAC CCCACGATAC 2041 TTAATTAAAA CACGCGGCCA GCAGGTCACA TTAAGTTGTT CTCCTATTTC TGGACATAGA 2101 AGCGTGTCCT GGTATCAGCA GACTCCAGGC CAAGGTTTGC AATTTCTCTT CGAGTATTTC 2161 AGTGAGACCC AGCGGAACAA GGGCAACTTT CCTGGGAGAT TCAGCGGGCG ACAATTCTCC 2221 AACTCACGGT CCGAAATGAA CGTCTCGACC CTGGAATTAG GGGACTCAGC ACTATACCTT 2281 TGTGCTTCAT CCTTAGAGGC AAGCTCTTAC AACAGCCCAC TTCATTTTGG AAATGGTACA 2341 CGATTGACAG TTACCGACAA AACTCACACT TGTCCCCAAT GTCCAGCCCC TGAGCTCCCA 2401 GGAGGGCCCT CTGTCTTTGT CTTCCCCCCG AAACCCAAGG ACGTCCTCTC CATTTCTGGG 2461 AGGCCCGAGG TCACGTGCGT TGTAGTGGAC GTCGGAAAGG AAGACCCCGA GGTCAATTTC 2521 AACTGGTATA TTGATGGCGT TGAGGTGCGA ACGGCCAATA CGAAGCCAAA AGAGGAACAG 2581 TTCAACAGCA CGTACCGCGT GGTCAGCGTC CTGCCCATCC AGCACCAGGA CTGGCTGACG 2641 GGGAAGGAAT TCAAGTGCAA GGTCAACAAC AAAGCTCTCC CGGCCCCCAT CGAGAGGACC 2701 ATCTCCAAGG CCAAAGGGCA AACCCGGGAG CCGCAGGTGT ACACCCTGGC CCCATGCCGG 2761 GAAGAGCTGG CCAAGGACAC CGTGAGCGTA TGGTGCCTGG TCAAAGGCTT CTACCCAGCT 2821 GACATCAACG TTGAGTGGCA AAGGAACGGT CAGCCGGAGT CAGAGGGCAC CTATGCCAAC 2881 ACGCCGCCAC AGCTGGACAA CGACGGGACC TACTTCCTCT ACAGCAAGCT CTCGGTGGGA 2941 AAGAACACGT GGCAGCGGGG AGAAACCTTA ACCTGTGTGG TGATGCATGA GGCCCTGCAC 3001 AACCACTACA CCCAGAAATC CATCTCCCAG TCTCCGGGTA AAGGGTACCA GGACTATGAG 3061 CCCGAAGCCT AATAGTCTAG ACCTAGGTGA TCATAATCAG CCATACCACA TTTGTAGAGG 3121 TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG 3181 CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 3241 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 3301 TCATCAATGT ATCTTATCAT GTCTGGAGAT CTCTAGCTAG AGGATCGATC CCCGCCCCGG 3361 ACGAACTAAA CCTGACTACG ACATCTCTGC CCCTTCTTCG CGGGGCAGTG CATGTAATCC 3421 CTTCAGTTGG TTGGTACAAC TTGCCAACTG AACCCTAAAC GGGTAGCATA TGCTTCCCGG 3481 GTAGTAGTAT ATACTATCCA GACTAACCCT AATTCAATAG CATATGTTAC CCAACGGGAA 3541 GCATATGCTA TCGAATTAGG GTTAGTAAAA GGGTCCTAAG GAACAGCGAT GTAGGTGGGC 3601 GGGCCAAGAT AGGGGCGCGA TTGCTGCGAT CTGGAGGACA AATTACACAC ACTTGCGCCT 3661 GAGCGCCAAG CACAGGGTTG TTGGTCCTCA TATTCACGAG GTCGCTGAGA GCACGGTGGG 3721 CTAATGTTGC CATGGGTAGC ATATACTACC CAAATATCTG GATAGCATAT GCTATCCTAA 3781 TCTATATCTG GGTAGCATAG GCTATCCTAA TCTATATCTG GGTAGCATAT GCTATCCTAA 3841 TCTATATCTG GGTAGTATAT GCTATCCTAA TTTATATCTG GGTAGCATAG GCTATCCTAA 3901 TCTATATCTG GGTAGCATAT GCTATCCTAA TCTATATCTG GGTAGTATAT GCTATCCTAA 3961 TCTGTATCCG GGTAGCATAT GCTATCCTAA TAGAGATTAG GGTAGTATAT GCTATCCTAA 4021 TTTATATCTG GGTAGCATAT ACTACCCAAA TATCTGGATA GCATATGCTA TCCTAATCTA 4081 TATCTGGGTA GCATATGCTA TCCTAATCTA TATCTGGGTA GCATAGGCTA TCCTAATCTA 4141 TATCTGGGTA GCATATGCTA TCCTAATCTA TATCTGGGTA GTATATGCTA TCCTAATTTA 4201 TATCTGGGTA GCATAGGCTA TCCTAATCTA TATCTGGGTA GCATATGCTA TCCTAATCTA 4261 TATCTGGGTA GTATATGCTA TCCTAATCTG TATCCGGGTA GCATATGCTA TCCTCATGAT 4321 AAGCTGTCAA ACATGAGAAT TAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT 4381 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA 4441 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC 4501 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT 4561 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT 4621 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT 4681 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT 4741 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC 4801 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC 4861 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT 4921 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG 4981 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG 5041 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA 5101 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA 5161 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT 5221 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC 5281 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG 5341 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT 5401 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT 5461 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC 5521 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 5581 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 5641 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 5701 TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 5761 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 5821 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 5881 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 5941 ACCTACACCG AACTGAGATA //