-- Parse module. -- By G.W. Schwartz -- {- | Collection of functions for the parsing of a fasta file. Uses the Text type. -} {-# LANGUAGE OverloadedStrings #-} module Data.Fasta.Text.Parse ( parseFasta , parseCLIPFasta , removeNs , removeCLIPNs ) where -- Built-in import Data.Char import qualified Data.Map as M import Control.Monad (void) import Text.Parsec import Text.Parsec.Text import qualified Data.Text as T -- Local import Data.Fasta.Text.Types eol :: Parsec T.Text u String eol = choice . map (try . string) $ ["\n\r", "\r\n", "\n", "\r"] eoe :: Parsec T.Text u () eoe = do lookAhead (void $ char '>') <|> eof fasta :: Parsec T.Text u FastaSequence fasta = do spaces char '>' header <- manyTill (satisfy (/= '>')) eol fseq <- manyTill anyChar eoe return (FastaSequence { fastaHeader = T.pack header , fastaSeq = T.pack . map toUpper . removeWhitespace $ fseq } ) where removeWhitespace = filter (`notElem` "\n\r ") fastaFile :: Parsec T.Text u [FastaSequence] fastaFile = do spaces many fasta fastaCLIP :: Parsec T.Text u (FastaSequence, [FastaSequence]) fastaCLIP = do spaces char '>' germline <- fasta clones <- many $ try fasta return (germline, clones) fastaCLIPFile :: Parsec T.Text u [(FastaSequence, [FastaSequence])] fastaCLIPFile = do spaces many fastaCLIP -- | Parse a standard fasta file into text sequences parseFasta :: T.Text -> [FastaSequence] parseFasta = eToV . parse fastaFile "error" where eToV (Right x) = x eToV (Left x) = error ("Unable to parse fasta file\n" ++ show x) -- | Parse a CLIP fasta file into text sequences parseCLIPFasta :: T.Text -> CloneMap parseCLIPFasta = M.fromList . map (\(x, (y, z)) -> ((x, y), z)) . zip [0..] . eToV . parse fastaCLIPFile "error" where eToV (Right x) = x eToV (Left x) = error ("Unable to parse fasta file\n" ++ show x) -- | Remove Ns from a collection of sequences removeNs :: [FastaSequence] -> [FastaSequence] removeNs = map (\x -> x { fastaSeq = noN . fastaSeq $ x }) where noN = T.map (\y -> if (y /= 'N' && y /= 'n') then y else '-') -- | Remove Ns from a collection of CLIP fasta sequences removeCLIPNs :: CloneMap -> CloneMap removeCLIPNs = M.fromList . map remove . M.toList where remove ((x, y), z) = ((x, newSeq y), map newSeq z) newSeq x = x { fastaSeq = noN . fastaSeq $ x } noN = T.map (\y -> if (y /= 'N' && y /= 'n') then y else '-')