#include #include "bam.h" #include "khash.h" typedef char *str_p; KHASH_MAP_INIT_STR(s, int) KHASH_MAP_INIT_STR(r2l, str_p) void bam_aux_append(bam1_t *b, const char tag[2], char type, int len, uint8_t *data) { int ori_len = b->data_len; b->data_len += 3 + len; b->l_aux += 3 + len; if (b->m_data < b->data_len) { b->m_data = b->data_len; kroundup32(b->m_data); b->data = (uint8_t*)realloc(b->data, b->m_data); } b->data[ori_len] = tag[0]; b->data[ori_len + 1] = tag[1]; b->data[ori_len + 2] = type; memcpy(b->data + ori_len + 3, data, len); } uint8_t *bam_aux_get_core(bam1_t *b, const char tag[2]) { return bam_aux_get(b, tag); } #define __skip_tag(s) do { \ int type = toupper(*(s)); \ ++(s); \ if (type == 'Z' || type == 'H') { while (*(s)) ++(s); ++(s); } \ else if (type == 'B') (s) += 5 + bam_aux_type2size(*(s)) * (*(int32_t*)((s)+1)); \ else (s) += bam_aux_type2size(type); \ } while(0) uint8_t *bam_aux_get(const bam1_t *b, const char tag[2]) { uint8_t *s; int y = tag[0]<<8 | tag[1]; s = bam1_aux(b); while (s < b->data + b->data_len) { int x = (int)s[0]<<8 | s[1]; s += 2; if (x == y) return s; __skip_tag(s); } return 0; } // s MUST BE returned by bam_aux_get() int bam_aux_del(bam1_t *b, uint8_t *s) { uint8_t *p, *aux; aux = bam1_aux(b); p = s - 2; __skip_tag(s); memmove(p, s, b->l_aux - (s - aux)); b->data_len -= s - p; b->l_aux -= s - p; return 0; } int bam_aux_drop_other(bam1_t *b, uint8_t *s) { if (s) { uint8_t *p, *aux; aux = bam1_aux(b); p = s - 2; __skip_tag(s); memmove(aux, p, s - p); b->data_len -= b->l_aux - (s - p); b->l_aux = s - p; } else { b->data_len -= b->l_aux; b->l_aux = 0; } return 0; } void bam_init_header_hash(bam_header_t *header) { if (header->hash == 0) { int ret, i; khiter_t iter; khash_t(s) *h; header->hash = h = kh_init(s); for (i = 0; i < header->n_targets; ++i) { iter = kh_put(s, h, header->target_name[i], &ret); kh_value(h, iter) = i; } } } void bam_destroy_header_hash(bam_header_t *header) { if (header->hash) kh_destroy(s, (khash_t(s)*)header->hash); } int32_t bam_get_tid(const bam_header_t *header, const char *seq_name) { khint_t k; khash_t(s) *h = (khash_t(s)*)header->hash; k = kh_get(s, h, seq_name); return k == kh_end(h)? -1 : kh_value(h, k); } int bam_parse_region(bam_header_t *header, const char *str, int *ref_id, int *beg, int *end) { char *s; int i, l, k, name_end; khiter_t iter; khash_t(s) *h; bam_init_header_hash(header); h = (khash_t(s)*)header->hash; *ref_id = *beg = *end = -1; name_end = l = strlen(str); s = (char*)malloc(l+1); // remove space for (i = k = 0; i < l; ++i) if (!isspace(str[i])) s[k++] = str[i]; s[k] = 0; l = k; // determine the sequence name for (i = l - 1; i >= 0; --i) if (s[i] == ':') break; // look for colon from the end if (i >= 0) name_end = i; if (name_end < l) { // check if this is really the end int n_hyphen = 0; for (i = name_end + 1; i < l; ++i) { if (s[i] == '-') ++n_hyphen; else if (!isdigit(s[i]) && s[i] != ',') break; } if (i < l || n_hyphen > 1) name_end = l; // malformated region string; then take str as the name s[name_end] = 0; iter = kh_get(s, h, s); if (iter == kh_end(h)) { // cannot find the sequence name iter = kh_get(s, h, str); // try str as the name if (iter == kh_end(h)) { if (bam_verbose >= 2) fprintf(stderr, "[%s] fail to determine the sequence name.\n", __func__); free(s); return -1; } else s[name_end] = ':', name_end = l; } } else iter = kh_get(s, h, str); *ref_id = kh_val(h, iter); // parse the interval if (name_end < l) { for (i = k = name_end + 1; i < l; ++i) if (s[i] != ',') s[k++] = s[i]; s[k] = 0; *beg = atoi(s + name_end + 1); for (i = name_end + 1; i != k; ++i) if (s[i] == '-') break; *end = i < k? atoi(s + i + 1) : 1<<29; if (*beg > 0) --*beg; } else *beg = 0, *end = 1<<29; free(s); return *beg <= *end? 0 : -1; } int32_t bam_aux2i(const uint8_t *s) { int type; if (s == 0) return 0; type = *s++; if (type == 'c') return (int32_t)*(int8_t*)s; else if (type == 'C') return (int32_t)*(uint8_t*)s; else if (type == 's') return (int32_t)*(int16_t*)s; else if (type == 'S') return (int32_t)*(uint16_t*)s; else if (type == 'i' || type == 'I') return *(int32_t*)s; else return 0; } float bam_aux2f(const uint8_t *s) { int type; type = *s++; if (s == 0) return 0.0; if (type == 'f') return *(float*)s; else return 0.0; } double bam_aux2d(const uint8_t *s) { int type; type = *s++; if (s == 0) return 0.0; if (type == 'd') return *(double*)s; else return 0.0; } char bam_aux2A(const uint8_t *s) { int type; type = *s++; if (s == 0) return 0; if (type == 'A') return *(char*)s; else return 0; } char *bam_aux2Z(const uint8_t *s) { int type; type = *s++; if (s == 0) return 0; if (type == 'Z' || type == 'H') return (char*)s; else return 0; } #ifdef _WIN32 double drand48() { return (double)rand() / RAND_MAX; } #endif